effects of beta-naphthoflavone and 3-methylcholantheren on biochemical markers in sturgeon fish, huso huso

نویسندگان

کتایون کریم زاده

katayoon karimzadeh department of marine biology, islamic azad university, branch of lahijan 1616, lahijan, iran.

چکیده

background: polycyclic aromatic hydrocarbons (pahs) are a predominant group of contaminants that have been shown to accumulate and persist in marine organisms. we evaluated the dose-dependent induction of the cytochrome p4501a (cyp1a) system in the sturgeon fish, huso huso after after exposure to pahs, beta-naphthoflavone (bnf) and 3- methylcholantheren. methods: the fish were treated by i.p. injections of beta–naphthoflavone and 3- methylcholantheren dissolved in corn oil at three doses 35, 70 and 105 mg/kg wet-body weight respectively for 72 h every day. similarly, the control fish received injections of only corn oil. microsomal fraction was prepared and ethoxy resorufin-o-deethylase activity (erod) was measured in the fish liver. cytochrome p4501a1 (cyp1a1) content was estimated by elisa based on monoclonal anti-cod p4501a1. results: beta-naphthoflovone and 3-methylcholantheren treated-fish developed 15-32 fold increase in ethoxyresorufin-o-deethylase activity in liver microsomes. the results of elisa showed higher levels of cytochrome p4501a content in treated fish as compared to controls. conclusion: a parallel increase of the cyp1a immunochemically assay and an increase in erod activity could be recorded for beta –naphthoflavone and 3- methylcholantheren in injected fish. dose-response relationships on the induction of erod and cyp1a immunodetectable protein were recorded. therefore, erod activity and cyp1a content can be used as a biomarker of polycyclic aromatic hydrocarbons.

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Effects of Beta-Naphthoflavone and 3-Methylcholantheren on Biochemical Markers in Sturgeon Fish, Huso huso

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عنوان ژورنال:
iranian journal of toxicology

جلد ۱۰، شماره ۳، صفحات ۱۳-۱۷

کلمات کلیدی
[ 3 , ' m e t h y l c o l a n t h e r e n ' , ' b e t a n a p h t h o f l a v o n ' , ' b i o m a r k e r ' , ' e t h o x y r e s o r u f i n d e e t h y l a s e ' , ' h u s o h u s o . ' ]

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